Our results indicate that the addition of both a 40- and 60-bp tail onto the original sequencing primer enhances the quality of the sequence, 1.2–4.6 times (Figure 1 and supplementary materials) (40 bp, P ≤ 0.01 60 bp, P < 0.01 paired Student's t-test and Wilcoxon signed rank test, respectively). No amplification products were observed in the controls. Extraction and PCR blank controls were incorporated at ratios of 1:8 and 1:1, respectively.
Gatherer microsynth full#
To prevent contamination, sample preparation, DNA extractions, and PCR setup were carried out in a dedicated ancient DNA facility physically isolated from other biological laboratories (including post-PCR facilities) with positive air pressure, daily exposure of surfaces to ultraviolet (UV)-irradiation, and where full body suits, face masks, and disposable gloves are worn. The PCR products were purified prior to DNA sequencing using the Invisorb® Vacuum Manifold and Invisorb PCR HTS 96 kit (both from Invitek GmbH, Berlin, Germany).
Cycling was performed in an Eppendorf® Mastercycler® gradient thermal cycler (Eppendorf Nordic, Horsholm, Denmark) using denaturation at 94☌ for 2 min, followed by 45–50 cycles of 94☌ for 30 s, 50°–55☌ for 30 s, and 68°–72☌ for 30 s, and a final cycle for 7–10 min at 68°–72☌. PCR amplification was performed using conventional primers (see Supplementary Table S2 for primer details) in 25-µL reactions containing 1× PCR High Fidelity PCR buffer, 2.5 mM magnesium sulfate solution (Invitrogen, Carlsbad, CA, USA), 0.4 mM dNTP mix, 1 U Platinum® Taq DNA Polymerase High Fidelity (Invitrogen), 1 µM each primer, and 1–5 µL DNA extract. Stack: Interface\AddOns\Gatherer\Astrolabe\Astrolabe.DNA was extracted from two bone specimens of the extinct Pleistocene woolly rhinoceros (Coelodonta antiqutatis) and from three bone specimens of Pleistocene musk oxen (Ovibos sp.) (sample details in Supplementary Table S1, available online at using conventional silica-based methods (7,8). Message: Interface\AddOns\Gatherer\Astrolabe\a:1286: attempt to call global 'GetMapContinents' (a nil value) (*temporary) = defined = "attempt to call field 'AddButton' (a nil value)" Stack: Interface\AddOns\Gatherer\Reports\a:24: attempt to call field 'AddButton' (a nil value) Message: Interface\AddOns\Gatherer\Reports\a:24: attempt to call field 'AddButton' (a nil value) If you want to configure the addon, click the Gatherer configuration icon floating around your minimap frame or type /gather options. When you gather things, they will appear on your minimap and your main map. Gatherer DB WoWHead - A database of node locations provided by INSTALLATIONĮxtract the data to your "World of Warcraft/Interface/AddOns" directory so that the "Gatherer" directory is a subdirectory of the "AddOns" directory. When you view your World Map, you will also see the item locations marked on the particular map you are viewing there.įor more information, please visit us at From then on, whenever the item comes into range of being one of the closest 1-25 (configurable) items to your present location, it will pop up on you minimap. It does this whenever you gather (perform herbalism, mining or opening) on an item, and records the specific map location in its history. The addon does not track like a tracking ability does, rather it "remembers" where you have found various items in the past. It's main purpose is to track the closest plants, deposits and treasure locations on your minimap. Gatherer is an addon for herbalists, miners and treasure hunters in World of Warcraft.
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